Most of the CID patients clearly segregated far from healthy donors, similarly to SCID (Figures 2A,B and Supplementary Figures 2, 5A) and the main discriminating variables were the TCM CD4+ and TEM CD8+ and to a lesser extent TEMRA CD8+ cell subsets, as clearly evident in the CID patients (C9, C13, C14, C15) diagnosed as APDS (activated PI3K delta syndrome) (Figure 2B). Here, CD8A is linked to combined immunodeficiency.