GFAP and Zinc deficiency: Our results show a significant increase in GFAP expression levels in brains of mice subjected to zinc deficiency and lowered bioavailability in the diet compared to control mice (one way ANOVA, p = 0.0044; Tukey post hoc analysis: Control vs. Zinc deficient, p = 0.0447; Control vs. Zinc inhibitor, p = 0.0036) (Figure 4A).