LATS1 and hepatocellular carcinoma: To assess whether LATS1-induced ubiquitination of Beclin-1 had a causal role in autophagy regulation, we re-introduced siRNA-refractory versions of either WT Beclin-1 or its K32/263R mutant into Beclin-1 knockdown cells (Fig. 6c) and assessed autophagy induction by Srf in HCC cells and by rapamycin in U2OS cells.