To further examine the role of LATS1 in Srf resistance in HCC, we established Srf-resistant Huh7 cells by (1) step-wise increasing the concentration of Srf to induce resistance (Huh7-IR cells) or (2) by adding a consistent high concentration of Srf to induce resistance (Huh7-CR cells) (Fig. 1e and Supplementary Fig. 1g, h). Here, LATS1 is linked to hepatocellular carcinoma.