The tumors and spleens belonging to mice inoculated with B16.F10 cells were collected, processed into single-cells suspension, and labelled with anti-(a)CD8 for T-cells, aCD19 for B-cells, gp100 and TRP-2 pentamers for tumor-specific T cells, aPD1 for antigen-experienced T-cells, aCD68 for macrophages identification, aCD11c for dendritic cells, and aCD370 for cross-presenting activated dendritic cells. The gene discussed is CD8A; the disease is neoplasm.