To investigate whether the miR-496–driven suppression of bladder cancer progression was mediated via direct targeting of HMGB1 mRNA, rescue experiments were conducted with T24 and TCC-SUP cells cotransfected with the miR-496 mimics and either the HMGB1-overexpressing plasmid pcDNA3.1-HMGB1 (hereafter: pc-HMGB1) or the empty pcDNA3.1 vector. The gene discussed is HMGB1; the disease is urinary bladder cancer.