To investigate whether NNT-AS1 is functionally implicated in the aggressiveness of bladder cancer, either small interfering RNAs (siRNAs) specific to NNT-AS1 (siNNT-AS1) or a negative control (NC) siRNA (siNC) were transfected into T24 and TCC-SUP cells, and the efficiency of siRNA transfection was assessed by RT-qPCR. The gene discussed is NNT; the disease is urinary bladder carcinoma.