To confirm the correlation between the degree of GPS1 protein downregulation and the decrease in virus titer, four kinds of siRNAs with different target sequences in the GPS1 gene (siRNA GPS1_2, siRNA GPS1_3, siRNA GPS1_5, and siRNA GPS1_6) were each transfected into HEK293 cells, and then virus infection and titration assessments were performed as in the previous experiments. The gene discussed is GPS1; the disease is viral infectious disease.