Shen et al. [69] described a simple method to conjugate multifunctional nanoparticles (MFNPs) assembled by the formation of various layers with a monoclonal anti-HER2 antibody and confirmed that the MFNPs exhibited the specific detection of breast cancer BT474 cells (biomarker HER2 positive) with a high signal-to-noise ratio. Here, ERBB2 is linked to breast carcinoma.