The data obtained in the present study suggest that the RASAL1/SCD1 axis might play an essential role in development of colon cancer cells, RASAL1 was able to inhibit the fatty acid synthesis by inhibit SCD1 3′-UTR activity via LXRα and SREBP1c pathway, then inhibit the cell proliferation by cell cycle arrest. This evidence concerns the gene SREBF1 and colonic neoplasm.