Increased levels of IL-1β were also detected using three-dimensional (3D) in vitro models of breast cancer bone metastases where different breast cancer cell lines were cultured with bone tissue fragments from non-osteoporotic [41,42] and osteoporotic patients [43]; this last study also showed a higher expression of IL-1β in comparison to non-osteoporotic patients [43]. The gene discussed is IL1B; the disease is breast carcinoma.