To explore the clinical significance of NELFA mRNA in ESCC, we obtained tissue microarrays including 93 ESCC and 67 para‐carcinoma tissues from Outdo Biotech Co. Then, RNAscope ISH assays were applied to detect NELFA mRNA expression through the tissue microarrays. The gene discussed is NELFA; the disease is esophageal squamous cell carcinoma.