EGFR and melanoma: Today, targeted sequencing is used in routine practice to identify hotspot activating mutations in BRAF or EGFR in melanoma or lung cancer, respectively.19,20 Unfortunately, in SCCOHT, SMARCA4 mutations are not ‘hot-spot’ and can occur anywhere along the whole gene (ENST00000344626, 5392 bp) with different mutation types including splice site, missense and frameshift making targeted sequencing difficult in routine care.