Today, targeted sequencing is used in routine practice to identify hotspot activating mutations in BRAF or EGFR in melanoma or lung cancer, respectively.19,20 Unfortunately, in SCCOHT, SMARCA4 mutations are not ‘hot-spot’ and can occur anywhere along the whole gene (ENST00000344626, 5392 bp) with different mutation types including splice site, missense and frameshift making targeted sequencing difficult in routine care. The gene discussed is BRAF; the disease is lung carcinoma.