To relevant natural existence of synergized PSPC1 and PTK6 oncogenic effects of the dynamic PSPC1/PTK6/β-catenin/Wnt3a axis in HCC, we generated an antibody against PSPC1-Y523 phosphorylation by using a phospho-peptide and validated its specificity (Supplementary Fig. 6a–c). Here, PSPC1 is linked to hepatocellular carcinoma.