PSPC1 and hepatocellular carcinoma: To determine the tumor suppressive effects of PSPC1-CT131, we constructed and ectopically expressed the PSPC1-CT131-EGFP fusion protein and its nuclear targeting mutant Mut-NLS-CT131 by disrupting the nuclear localization sequence (NLS) in HCC cell line Mahlavu (Supplementary Fig. 7b).