After altering the expression of EZH2 in U87 cells, we employed RT-qPCR and Western blot analysis (Fig. 2a–c), which showed increased EZH2 expression in glioma cells treated with oe-EZH2, whereas decreased EZH2 expression was noted in U87 cells treated with sh-EZH2 (p < 0.05). Here, EZH2 is linked to glioma.