MLH1 and cancer: As expected, hMLH1 was downregulated and hypermethylated in the distal promoter in Co115, to a lesser extent in HT29 but not in Colo205 (Fig. 3b, c), consistent with the previously shown heterogeneity of hMLH1 silencing in BRAFV600E CIMP cancers and SSA/Ps [13, 27, 28].