To further expand these observations, we have selected other two CRC cell lines sensitive to MEK blockade, such as HCT116 (KRAS G13D) and LIM1215 (all RAS WT), to generate by an in vitro selection new models of acquired resistance to MEKi (Fig. 2, Additional file 1: Figure S1). Here, MAP2K7 is linked to colorectal carcinoma.