RNAseq data analysis showed that more than 2000 genes are differentially expressed in two USP22−/− cancer cells (Additional file 1: Figure S1), and about 300 and 484 genes were unanimously upregulated or downregulated in two USP22−/− cancer cells (P < 0.01, false discovery rate: FDR < 0.05, log2 fold change: log2 FC ≥ 1, Additional file 2: Table S1). The gene discussed is USP22; the disease is cancer.