We firstly reported five novel mutations associated with autosomal dominant cataracts: c.154 T > C in GJA8, c.1152_1153insG in GJA3, c.1804G > C in BFSP1, c.1532C > T in EPHA2, c.356G > A in HSF4. This study expands the mutation spectrum of congenital cataracts, and provide solid evidence for genetic counseling and prenatal gene diagnosis of the cataract families. This evidence concerns the gene HSF4 and Autosomal dominant optic atrophy and cataract.