In summary, we performed genetic analysis in five Chinese families with congenital dominant cataracts and identified five novel mutations, including an insertion mutation encoding p.S385Efs*83 in GJA3 and four missense mutations: p.F52 L in GJA8, p.G602R in BFSP1, p.T511 M in EPHA2 and p.R119H in HSF4. This work extended the mutation spectrum of congenital cataracts, and would provide more evidences for the precise diagnosis of the disease. This evidence concerns the gene HSF4 and early-onset non-syndromic cataract.