We reported five novel mutations associated with the autosomal dominance cataract in five Chinese families respectively: c.154 T > C in GJA8, c.1152_1153insG in GJA3, c.1804G > C in BFSP1, c.1532C > T in EPHA2 and c.356G > A in HSF4. All of the five mutations were screened by targeted NGS for the 38 candidate genes of congenital cataracts, and verified through Sanger DNA sequencing. The gene discussed is GJA8; the disease is early-onset non-syndromic cataract.