We reported five novel mutations associated with the autosomal dominance cataract in five Chinese families respectively: c.154 T > C in GJA8, c.1152_1153insG in GJA3, c.1804G > C in BFSP1, c.1532C > T in EPHA2 and c.356G > A in HSF4. All of the five mutations were screened by targeted NGS for the 38 candidate genes of congenital cataracts, and verified through Sanger DNA sequencing. This evidence concerns the gene GJA3 and early-onset non-syndromic cataract.