This study for the first time highlights the tumor-suppressive role of LncRNA MAGI2-AS3 in HGSC and demonstrates its involvement in the regulation of miR-15b-5p, miR-374a-5p, and miR-374b-5p, and of their downstream mRNA targets (HOXA5, MTSS1, PTEN, and RECK) in HGSC cell lines. Here, PTEN is linked to neoplasm.