Consistent with our observations in human cancer lines, Pck1 depletion decreased murine CRC cell liver colonization in an immune competent model (p=0.039, p=0.005 for shCTRL vs shPck1-64, shCTRL vs shPck1-66, respectively) and did not impair in vitro proliferation under basal cell culture conditions (Figure 5E). Here, PCK1 is linked to colorectal carcinoma.