Similar to PCK1 inhibition by shRNA, pre-treatment of cells with 3 MPA significantly reduced (p=0.01) mCRC liver colonization in vivo (Figure 7A, Figure 7—figure supplement 1A), Pre-treatment of LS174T cells with 3 MPA did not, however, alter subcutaneous tumor growth (Figure 7—figure supplement 1B). The gene discussed is PCK1; the disease is neoplasm.