Lastly, given the significance of the IFN-γ pathway to anti-tumor immune responses and to determine whether BRCA1-deficient and normal tumors differed in type of immune cell infiltrates, we used TIMER to predict the relative numbers of immune cells in tumor specimens based on gene expression data.34 Predicted numbers of six immune cell subtypes (B cells, CD4, CD8, macrophages, neutrophils, and dendritic cells) are shown in Supplementary Fig. 9. This evidence concerns the gene BRCA1 and neoplasm.