To evaluate whether in silico analyzed novel genes directly regulated by the NRF2 transactivation, we selected the five novel NRF2 regulated genes in KMSGC such as NEIL3, TSPAN7, CBX2, UCHL1, and TRIM16L along with bonafide NRF2 genes-AKR1C1, NQO1, G6PD and performed qRT PCR analysis in NRF2 knocked down A549 NSCLC cells as described previously 12. Here, AKR1C1 is linked to non-small cell lung carcinoma.