Therefore, given the regulation of miR221/p27/DNMT1 signaling pathway, we designed the gold nanoparticles (AuNPs) loaded with anti-miR221, a specific inhibitor of miR221, to activate the expression of p27 and suppress the expression of DNMT1, which could finally enhance the effect of sorafenib in HCC. Here, DNMT1 is linked to hepatocellular carcinoma.