hASH1 localisation appears to be sensitive to androgen signalling; AD caused marked hASH1 accumulation and nuclear localisation in LNCaP cells, whilst prominent hASH1 staining and nuclear localisation was found in androgen-insensitive, DU145 and PC3 cells under control, unstripped conditions (Fig. 3). This evidence concerns the gene ASCL1 and Alzheimer disease.