Nanoparticles modified with the more potent truncated tLyp-1 peptide reduced immunosuppressive Tregs in the TME of murine B16/BL6 melanoma tumours in vivo, while activating intratumoural CD8+ T cells when combined with an anti-CTLA-4 immunotherapeutic (Figure 1). This evidence concerns the gene CD8A and melanoma.