In summary, from this study, we obtained some novel findings: (1) acupuncture on the three acupoints, ST36, CV4 and KI1, can improve pathological process of NAFLD; (2) acupuncture treatment can inhibit inflammatory reactions, reduce oxidative stress and promote lipid metabolism; (3) acupuncture on different acupoints can inhibit inflammatory reactions by regulating specific cytokines; (4) acupuncture treatment have regulatory effects on the expression of transcription factors. The gene discussed is TNFRSF8; the disease is metabolic dysfunction-associated steatotic liver disease.