Taken together, these results indicate that GLTSCR1 may negatively regulate the transcriptional elongation of target oncogenes to inhibit CRC metastasis through interacting with BRD4 to inhibit CDK9 to phosphorylate RNA Pol II at Ser2 and to compete with SCP2 to maintain the phosphorylation of RNA Pol II at Ser5. Here, CDK9 is linked to colorectal carcinoma.