BICRA and colorectal carcinoma: Then, polymerase chain reaction (PCR) amplification combined with DNA sequencing was used to further verify heterozygous frameshift mutations with the deletion or insertion of a single cytosine nucleotide in the GLTSCR1 C8 microsatellite site in these CRC samples (Figure 1B); these results were reconfirmed by capillary electrophoresis (Figure 1C).