As a chromatin adapter, BRD4 can recognize acetylated H3 K14 or H4 K5 or K12 histone marks by its bromodomain23 and modulate RNA polymerase II (Pol II)‐mediated transcriptional elongation through positive regulation of pTEFb.24 To explore the molecular basis of the interaction between GLTSCR1 and BRD4 in CRC metastasis suppression, we performed genome‐wide transcriptional analysis in two HCT116 GLTSCR1‐KO cell lines by RNA sequencing (RNA‐seq). Here, BRD4 is linked to colorectal carcinoma.