Therefore, we employed extended comparison methods (Supplementary Table 5) to assess presence versus absence and there was emergence of weak intensity of epithelial nuclear RUNX3 in dysplastic BO, compared with normal mucosa (p = 0.013), non-dysplastic BO (p = 0.001) and oesophageal adenocarcinoma (p = 0.002). This evidence concerns the gene RUNX3 and esophageal adenocarcinoma.