Furthermore, TqPCR analysis revealed that BMP4 injections effectively affected the expression of lipid synthesis and storage genes (Figure 4C, a and c) and lipid breakdown genes (Figure 4C, b and d) at both weeks 4 and 12, suggesting that exogenous BMP4 may significantly impact the dysregulated lipid metabolism during the development of NAFLD. The gene discussed is BMP4; the disease is metabolic dysfunction-associated steatotic liver disease.