TP53 and colorectal carcinoma: In different p53 status CRC cell lines, the single exposure (24 h) to either 1,25D3 (10, 50, 100, 500, and 1000 nM) or metformin (1, 2, 5, 7.5, 10, and 20 mM) reduces the cell viability in HCT116 (p53 wild-type), HCT116 (p53−/−), and HT-29 (p53 mutant) [43].