Moreover, TGF‐β1 signalling regulates EMT‐related genes, thereby facilitating cancer progression in CRC.10, 11 Towards this end, our research has focused on Krüppel‐like factor 4 (KLF4), a transcription factor that was involved in regulation of cell proliferation, apoptosis, differentiation and cancer progression.12, 13 In CRC cells, we found that KLF4 acted as an oncogene in colorectal CSCs‐enriched spheroid cells.14 However, in a CSCs model, it needs further investigation of the underlying mechanisms of KLF4, stemness and EMT. This evidence concerns the gene KLF4 and colorectal carcinoma.