To explore drugs that may act against KSHV and related cancer cells through targeting LANASIM, we developed a luciferase based live-cell reporter assay (containing two subunits of SmBiT and LgBiT) to quantitatively measure the interaction of LANASIM with SUMO2 via NanoLuc Binary Technology (Fig 1A). The gene discussed is SUMO2; the disease is cancer.