These differences were greater in tumours with a POLE mutation outside the exonuclease domain and concomitant MSI, which had a median TMB of 48.5 mut/Mb (> 100 mut/Mb in 6/17 cases); C>A and T>G proportions of 9.9% and 1.6%, respectively; a C>G frequency of 0.9%; and a median indel proportion of 14.5%. Here, POLE is linked to neoplasm.