To determine whether the ALDH2 inactivating mutation further increases mitochondrial dysfunction observed in AD patient-derived cells, we transiently transfected the fibroblasts of four healthy (control) subjects and of four ApoE-associated AD patients with the dominant mutant ALDH2*2 using lipofectamine ([59]; see Table 1 in Supplement material for further information about the cell lines). This evidence concerns the gene APOE and Alzheimer disease.