Since EMT was essential for driving plasticity during the migration of tumor cells, then we detected the expression change of E-cadherin and Vimentin through IF and western blot in HCC cells under hypoxic condition and found that suppression of SMC4 could upregulate the expression of E-cadherin but downregulate the expression of Vimentin (Figure 2(b)). This evidence concerns the gene SMC4 and hepatocellular carcinoma.