We aimed to investigate the effects of Nrf2 on drug sensitivity, thioredoxin reductase (TrxR) expression, reactive oxygen species (ROS) production, and apoptosis induction in imatinib-resistant CML K562/G01 cells and explored their potential mechanisms. The gene discussed is NFE2L2; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.