Meanwhile, RB (4,5,6,7-tetrachloro-2′,4′,5′,7′-tetraiodofluoresceindisodium) is used as the chemotherapeutic drug which is a water-soluble, photosensitive, synthetic dye used for diagnostics exhibiting cytotoxicity in various cancer types, such as brain cancer (Tserkovsky et al., 2012), colorectal cancer cells (Qin et al., 2017), melanoma and breast cancer cells (Toomey et al., 2013), and ovarian and adenovirus-transformed embryonic kidney cancer cells (Koevary, 2012). The gene discussed is RB1; the disease is brain cancer.