While the more common BRAF V600E and BRAF-KIAA1459 fusion mutations can be detected through non-sequencing technologies such as fluorescence in situ hybridization (FISH) or immunohistochemistry, these techniques will not detect the less common mutations observed in low-grade gliomas such as EGFR mutations or less common BRAF mutations. The gene discussed is BRAF; the disease is central nervous system cancer.