To address the translational barrier of measuring anti-influenza antibody-mediated immunity, we have previously developed a Luminex-based multiplex assay (mPlex-Flu assay) that can simultaneously measure absolute antibody concentrations (IgG, IgM, or IgA) against up to 50 influenza strains using ≤5 μL of serum [7,8,11,12]. The gene discussed is CD40LG; the disease is influenza.