The results showed that snail could activate the expression of CXCL8 directly by binding to the E3/E4 E-boxes, a CXCL8 promoter region, and then regulate colon cancer stem cell activity and EMT in the cancer cells, which indicated that CXCL8 can interact with snail to induce EMT and promote the invasion and metastasis of CRC cells. This evidence concerns the gene CXCL8 and malignant colon neoplasm.