We next explored the general effect of knocking down FTO in Hela and SiHa cells, as revealed by polysome profiling, the global translation of FTO deficient cervical cancer cells was comparable to FTO competent controls (Fig. 5c), however, when we evaluated the translation of E2F1 and Myc specifically, we found the translation efficiency of E2F1 and Myc was dramatically impaired after inhibiting FTO (Fig. 5c), resulting decreased protein level of E2F1 and Myc in cervical cancer cells (Fig. 5d). This evidence concerns the gene E2F1 and cervical carcinoma.