An illustrative case of the utility of unbiased parallel sequencing followed by segregation analysis vs candidate-gene direct sequencing in interpreting single variants is highlighted by the case of 2 affected brothers with CMT1 who were previously given a diagnosis of CMT1C due to a novel c.115C>T p.(Pro39Ser) mutation in LITAF identified by Sanger sequencing. The gene discussed is RNMT; the disease is Charcot-Marie-Tooth disease type 1.