CACNA1C and amyotrophic lateral sclerosis: This verifies that ALS-T2DM-IgGs are capable of directly interacting with CaVα2δ1 subunits in living cells in the absence of interferences from detergents, high ionic strengths, and substantial rinsing, which are unavoidable in immunoprecipitation and immunoblot analyses of association between ALS-T2DM-IgGs and CaV1.2 subunits.