A number of proteins including SORBS1 and MAP4K4 had multiple noncanonical isoforms that were detectable across multiple tissues, whereas the protein with the most isoforms identified was titin (Figure 3A), which is also the largest protein in the human genome with the most exons and whose splicing has been widely implicated in congenital heart diseases (Guo et al., 2012; Herman et al., 2012). The gene discussed is MAP4K4; the disease is congenital heart disease.