We examined DEPDC1B expression in rhabdomyosarcoma tumours using published RNA‐Seq data sets (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE28511), profiling ARMS and ERMS alongside control skeletal muscle and tumour adjacent skeletal muscle. This evidence concerns the gene DEPDC1B and rhabdomyosarcoma.