As we proved here by several methods, TGFBR1 is a functional downstream target of miR-4666-3p, and alterations in miR-4666-3p and TGFBR1 expression could affect the stemness of colon cancer cells and activation of TGF-β/Smad pathway. This evidence concerns the gene TGFBR1 and malignant colon neoplasm.