Furthermore, secreted levels of these cathepsins may serve as robust diagnostic markers, with an AUC of 77% and a sensitivity of 72% for CTSL and an AUC value of 82% with a sensitivity of 83% for CTSB, in distinguishing GBC patients from both chronic cholecystitis with benign gallbladder disease and healthy individuals. This evidence concerns the gene CTSL and Cholecystitis.