The expression of WT MYO5B inMYO5B-KD cells restored microvilli, while the expression of MYO5B–P660L, an MVID-associated mutation found within the Navajo population (that cannot bind to RAB11A), induced the formation of MIs but did not rescue theMYO5B-KD phenotype. This evidence concerns the gene MYO5B and microvillus inclusion disease.