Nkx2-5 is one of the master transcription factors of heart development that regulates cardiac ion channels.72,73 This gene was identified as the first gene involved in CHD by genetic association studies in large families.20,74 We determined the frequency/distribution of Nkx2-5 mutations and evaluated these mutations by using computational tools (Mutation Taster, SIFT, Polyphen2, PROVEAN and CADD). Here, NKX2-5 is linked to coronary artery disorder.