Figure 4 shows the expression of each gene in the sample. Among the NKPF category, NFKB1, REL, and RELA are found to be upregulated, with FC ≥ 1.2 in the RA sample, whereas NFKB2 and RELB are downregulated, with FC ≤ −1.2. The most upregulated gene is REL, with FC of 3.04, and the most downregulated gene is KPNA1, with FC of −1.85. It is interesting to note that the most upregulated gene, REL, falls into the NKPF group, whereas most downregulated gene, KPNA1, comes from the seed gene category. Here, NFKB1 is linked to rheumatoid arthritis.