coli was fed together with fish fodder to zebrafish and grouper for 30 days, the survival rates against V. vulnificus (204) was enhanced in a dose dependent manner along with immunomodulatory effect which can be observed with the increase in TNF-1 in grouper, TLR4, IL-1, NOS2, and NF-κB in zebrafish within 96 h post-infection. This evidence concerns the gene IL1B and infection.